84-1116-84　CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1, CX3C Chemokine Receptor 1, C-X3-C CKR-1, CX3C CKR-1, CMK-BRL-1, CMKBLR1, CMKDR1, Fractalkine Receptor, GPR13, GPRV28, V28) 100ug　C8299-01G

特徴

• CX3CR1 is a 40kD member of the delta-group of rhodopsin GPCRs.
• It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells.
• CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes.
• Mouse CX3CR1 is a 7-transmembrane protein that is 354aa in length.
• It contains a 32aa N-terminal extracellular region that shows no glycosylation, and a 56aa C-terminal cytoplasmic domain.
• Over aa1-32, 91-104, 169-196 and 258-274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively.
• Applications:Suitable for use in Flow Cytometry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Flow Cytometry: 2.5ug labels 10e6 cells.
• Validated with M1 mouse myeloid leukemia cell line
• Western Blot: 1ug/ml detected CX3CR1 in mouse myeloid leukemia cell line and NK-92 human natural killer lymphoma cell lineOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.