BRIX (Biogenesis of ribosomes in Xenopus; also BRIX domain-containing protein 2) is a 41kD (predicted) member of the BXDC2 family of proteins.
It is found in nucleoli, and appears to participate in the formation of a complex associated with pre-rRNA and enzymes that generate 60S ribosomal subunits.
Human BRIX is 353 amino acids in length.
It is characterized by the presence of a BRIX domain that lies between aa 60-249.
There is one splice variant that shows a 17aa substitution for aa11-27.
Over aa131-353, human BRIX shares 93% aa identity with mouse BRIX.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol.
Specificity:Recognizes human BRIX. Species sequence homology: mouse (93%).
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:E. coli-derived, recombinant human BRIX (aa131-353).