The metabotropic glutamate receptors have been organised into three groups.
mGluR5 and mGluR1 make up group I which, when activated, stimulates phospholipase C and activates protein kinase C to hydrolyse phosphoinositide phospholipids in the cell membrane.
Group I mGluRs, but not other groups, are activated by 3, 5-dihydroxyphenylglycine (DHPG).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Detects bands of ~125kD (monomer) and 250kD (dimer) on lysate from transfected HEK293 cells.
It has also been reported to dtecst bands at ~120kD (reduced) and 210kD (non-reduced) in protein extracts from adult rat cerebellum
Immunohistochemistry (Frozen): 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.01% BSA, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes rat metabotropic glutamate receptor 1.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Rt
Immunogen：Peptide corresponding to aa1115-1130 from the C-terminal region of rat mGluR1a.
