84-1116-77　PARC (p53-associated Parkin-like Cytoplasmic Protein, H7AP1, KIAA0708, UbcH7-associated Protein 1) 50ug　P3109-65G

特徴

• PARC (p53 associated parkin like cytoplasmic protein) antibody directly interacted and formed an approximately 1MD complex with p53 in the cytoplasm of unstressed cells.
• In the absence of stress, inactivation of PARC induced nuclear localization of endogenous p53 and activated p53 dependent apoptosis.
• Overexpression of PARC promoted cytoplasmic sequestration of ectopic p53.
• Furthermore, abnormal cytoplasmic localization of p53 was observed in a number of neuroblastoma cell lines; RNA interference-mediated reduction of endogenous PARC significantly sensitized these neuroblastoma cells in the DNA damage response.
• These results revealed that PARC is a critical regulator in controlling p53 subcellular localization and subsequent function.
• Applications:Suitable for use in Immunohistochemistry and Western Blot.
• Other Applications not tested.
• Recommended Dilution:Immunohistochemistry: 5ug/ml (Paraffin Embedded Only).
• Western Blot: 1–2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.