The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen.
Recently a number of interesting molecules have been identified that may be exploited as markers for lymphatic endothelium, including the hyaluronan receptor LYVE1, PALE, VEGFR3, and podoplanin.
LYVE1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall.
The deduced amino acid sequence of LYVE1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module, the prototypic HA binding domain of the Link protein superfamily.
Like CD44, the LYVE1 molecule binds both soluble and immobilized HA.
However, unlike CD44, the LYVE1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels.
Hence, LYVE1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Applications: Suitable for Western Blot and Immunohistochemistry.
Not suitable for human in Western Blot.
Other Applications not tested.
Recommended Dilutions:Western Blot: 8ug/ml.
Tested with mouse spleen lysates.
Not suitable for human in Western BlotImmunohistochemistry: ≥1:100.
Citrate buffer antigen retrieval is recommended
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as liquid in Tris-glycine, pH 7.5, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes mouse LYVE-1at ~45kD. Species Crossreactivity: human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa250-318 from the C-terminal region of murine LYVE-1.
