Using anti-Yo antibodies, three genes have been cloned (CDR 1-3).
CDR2 Cerebellar degeneration related protein 2) suppresses the transcriptional activity and DNA binding of nuclear factor-kappa B by an unknown mechanism.
The neuron specific expression of CDR2 is regulated by a post-transcriptional mechanism.
CDR2 is an onconeuronal antigen; i.e.
an antigen that is expressed by systemic tumors and elicits an immune response that may develop into an autoimmune neuronal degeneration.
Paraneoplastic cerebellar degeneration (PCD) is a disorder in which breast or ovarian tumors express CDR2, which normally is expressed in cerebellar Purkinje neurons
Applications:Suitable for ELISA, Immunohistochemistry and Western Blot.
Other Applications not tested.
Recommended Dilution:Wester Blot: 2ug/ml; Positive Control: HeLa whole cell lysates.
A band is seen at ~51kD
Immunohistochemistry: 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in Tris-citrate/phosphate, pH 7- 8, 0.09% sodium azide.
Specificity：Recognizes human CDR2 Species Crossreactivity: Bovine, mouse, primate.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Hu Mk Mo
Immunogen：A synthetic peptide made to a C-terminal region of the human CDR2.
