Transforming acidic coiled-coil containing protein 3 (TACC3; also know as ERIC-1) is a 92-140kD member of the TACC family of transcriptional regulatory proteins.
It is expressed in a variety of cell types, including Sertoli cells, endothelial cells, erythroid progenitors, and thyroid epithelium.
When phosphorylated by Aurora A on Ser558, it stabilizes the mitotic spindle.
It also interacts with nuclear histone acetyltransferases, promoting their positive effect on transcription.
Human TACC3 is 838 amino acids in length and contains one poly-Serine region (aa155-160) and a TACC coiled-coil domain (aa637-837).
There is one potential splice variant that shows a Lys substitution for aa102-462.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using Jurkat human acute T cel leukemia cell line and K562 human chronic myelogenous leukemia cell line
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1904-28C: IgG, H&L (HRP) Pab Dk x GtI1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab Dk x GtI1904-28K: IgG, H&L (HRP) Pab Rb x GtI1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab Rb x Gt
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes endogenous human TACC3 at ~130kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu689-Ile838 of human TACC3, expressed in E. coli (Accession #Q9Y6A5). Species Sequence Homology: mouse, 79%
