84-1116-72　SOX17 (SRY-Box Containing Gene, Sex Determining Region Y-Box 17, SRY, Transcription Factor SOX17) (NL®557) 500ul　S5364-45A

特徴

• SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain.
• SOX17 is suggested to play an important role in endoderm development.
• Applications:Suitable for use in Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunocytochemistry: 1:10: Tested using definitive endoderm differentiated BG01V cells.
• Cells were fixed in PBS containing 4% paraformaldehyde, and blocked with PBS containing 10% normal donkey serum, 0.1% Triton® X-100, and 1% BSA.
• After blocking, cells were incubated with S5364-45A (1:10) in blocking buffer for 3 hours in the dark.
• Between each step, cells were washed with PBS containing BSA.
• If a staining volume of 20 tests can be done in a staining volume of 250ul; 100 tests can be done in a staining volume of 50ul
• Optimal dilutions to be determined by the researcher.
• Absorption:557nm
• Emission:574nm
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• NorthernLights 557 conjugates are sensitive to light.