In vitro, the metalloprotease MetAP-2 catalyzes the hydrolysis of N-terminal methionine residues.
MetAP-2 is highly conserved across species.
The catalytic domains of S.
cerevisiae and human MetAP-2 proteins are ~80% homologous.
The function of MetAP-2 in vivo remains to be determined.
MetAP-2 has been identified, purified and cloned from rat cells as a 67kD eIF-2-associated (elongation inhibitory factor-2) protein, suggesting a possible role in protein translation.
Provocative experiments suggest MetAP-2 may also be involved in angiogenesis, the process of new blood vessel formation.
In these studies, MetAP-2 was determined to be the major cellular protein covalently bound to the potent anti-angiogenic agent fumagillin, following fumagillin treatment of HUVEC (human umbilical endothelial cells) cells or calf brain homogenates.
Fumagillin was able to selectively inhibit the growth of a MetAP-2-dependent yeast strain.
The fungal derived fumagillin, and its more potent metabolite TNP-470 (now in clinical trials), are effective at limiting both the size and metastasis of solid tumors.
TNP-470 inhibits neovascularization via endothelial cell cycle arrest in the late G1 phase.
The details of this process are not well understood.
In view of the identification of MetAP-2 as a fumagillin binding protein, future studies with MetAP-2 should provide important new insights into the mechanisms of fumagillin/TNP- 470 induced cell-cycle arrest and the role of MetAP-2 in angiogenesis.
Applications:Suitable for use in ELISA, Western Blot, and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol.
Specificity：Recognizes human METAP2.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Sf 21-derived, recombinant human Methionine Aminopeptidase 2 (aa2-478).
