AFP (a-Fetoprotein) is a 69-73kD member of the ALB/AFP/VDB family of proteins.
It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids.
Mature mouse AFP is 587 amino acids in length.
It contains three albumin domains (aa20-201, 208-393 and 400-591), plus 15 intrachain disulfide bonds.
Rat AFP contains an alternate start site at Met287 that may generate an intracellular 37kD form.
Mouse AFP also shows an analogous site at Met281 that may produce an equivalent isoform.
Over aa19-605, mouse AFP shares 83% and 66% aa identity with rat and human AFP, respectively.
Applications:Suitable for use in Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml; Tested on mouse plasma and rat placenta
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse alpha Fetoprotein. Species Crossreactivity: rat. Crossreacts ~10% with human alpha Fetoprotein.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to Lys19-Val605 from mouse alpha Fetoprotein, expressed in NS0 cells. Species Sequence Homology: rat; 83%, human; 66%
