Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate.
Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome.
Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase.
A splice variant of this gene has been described.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:16,000Western Blot: 0.1-0.3ug/ml detects a band at ~60kD in human liver lysates
Immunohistochemistry: 3-5ug/ml shows lysosomal staining of trophoblasts in paraffin embedded human placentaOptimal dilutions to be determined by the researcher.
Positive Control:Human Liver lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography
Form：Supplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes isoform a (NP_000193.1) of human IDS at ~60kD.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-KHFRFRDLEEDP, from the internal region of human IDS (NP_000193.1)
