EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
Forms either a homodimer or a heterodimer with a related family member.
Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Peptide ELISA: 1:64,000Western Blot: 0.3-0.1ug/ml detects a band at ~75kD observed in human Adipose lysates (calculatd MW: 64.5kD according to NP_076870.1) Immunoprecipitation: reported to immunoprecipitate EBF1 from induced 3T3-L1 expressing FLAG-EBF1Optimal dilutions to be determined by the researcher.
Positive Control:Human Adipose lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide, 0.5% BSA.
Specificity：Recognizes human EBF1 at ~75kD. Does not cross-react with EBF2, EBF3 and EBF4. Species sequence homology: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-HNQLPALANTSV, from the internal region of human EBF1 (NP_076870.1).
