Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form.
The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands.
These ligands cause proteolysis of Notch to liberate the intracellular domain.
Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC).
Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT).
This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL.
The notch family members are involved cell differentiation and development.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:30,000-1:90,000Western Blot: 1:500-1:1500 in 10ug of NIH/3T3 lysatesImmunohistochemistry (Paraffin): 1:100 Requires HIER using sodium citrate buffer pH6.0Optimal dilutions to be determined by the researcher.
Positive Control:NIH/3T3 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human Notch 2, cleaved N-terminal. This epitope is exposed only after gamma secretase cleavage and is not accessible in the uncleaved form. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide from the N-terminal sequence of the cleaved N intracellular domain (NICD) human Notch 2. Species sequence homology: rat (14/15)
