Glycine receptor a3 subunit, also known as GLRA3, belongs to the ligand-gated ion channel family.
Glycine functions as a inhibitory neurotransmitter throughout the CNS of mammals.
The Glycine receptor is a neurotransmitter ligand-gated ion channel.
GLRA3 is a pentamer that is composed of ligand binding alpha and structural beta subunits.
When glycine binds to the receptor, chloride conductance increases which produces hyperpolarization.
It is expressed in the hippocampus, olfactory bulb, and cerebellum.
GLRA3 may be involved in human and murine neurological disorders.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml detected glycine receptor alpha 3 subunit on 10ug of fetal human brain tissue lysate.
Immunohistochemistry (Paraffin): 1:100-1:500 detected Glycine Receptor alpha 3 subunit in mouse spinal cord and mouse eye tissue.
HIER using sodium citrate buffer pH 6.0Optimal dilutions to be determined by the researcher.
Positive Control:Fetal human brain tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Glycine Receptor a3 subunit at ~48kD. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide from the C-terminus of human GLRA3, conjugated to KLH. Cellular Localization: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Species Sequence Homology: rat, 92%
