84-1116-08　CDT1 (CDT, CDT-1, Chromatin Licensing and DNA Replication Factor 1, DUP, hDUP, human homolog of Drosophila Double Parked, RIS2) 100ul　C2300-01A

特徴

• CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery.
• It plays an important role in insuring that there is only one round of DNA replication per cell cycle.
• CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E.
• 2004).
• It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E.
• 2004).
• In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it.
• CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 was used to detect CDT1 in Jurkat cell lysate
• Optimal dilutions to be determined by the researcher.
• Positive Control:Jurkat Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.