VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein 34 and Cell surface antigen 33) is a 55-70kD member of the JAM family of Ig-Superfamily proteins.
It shows expression on gastric epithelium and testicular germ cells and likely participates in cell adhesion.
Mouse VSIG1 precursor is 407 amino acids in length.
It is a type I transmembrane glycoprotein that contains a 211aa extracellular domain (ECD) (aa23-234) and a 152aa cytoplasmic region.
The ECD contains one V-type (aa23-134) and one C2-type Ig-like domain (aa145-229).
Over aa23-234, mouse VSIG1 is 94% and 81% aa identical to rat and human VSIG1, respectively.
There is one potential alternate start site at Met108.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml detects VSIG1 in recombinant mouse and rat VSIG1Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 2.5mg BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Specificity：Recognizes the extracellular domain of mouse VSIG1. Species Crossreactivity: rat. Shows ~10% crossreactivity with recombinant human VSIG1 and <1% crossreactivity with recombinant mouse VSIG2, 3 and 4 and recombinant human VSIG3.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to Val23-Glu234 from extracellular domain of mouse VSIG1, expressed in NS0 cells. Species Sequence Homology: rat; 94%, human; 81%
