Semaphorin 4B (Sema4B; also SemaC) is a 95-100kD, class IV member of the Semaphorin family of proteins.
It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses.
Mature human Sema4B is a type I transmembrane glycoprotein that is 794 amino acids in length.
It contains a 674aa extracellular region (aa39-832) that is characterized by one Sema domain (aa65-502), a PSI region (aa520-574), and an Ig-like C2-type domain (aa599-658).
There is one potential soluble splice variant that shows a 29aa substitution for aa707-832.
Over aa39-712, human Sema4B shares 86% aa identity with mouse Sema4B.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.2um filtered solution in PBS, BSA. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA. Labeled with Biotin.
Specificity：Recognizes human Semaphorin 4B.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：NS0-derived recombinant human Semaphorin 4B aa39-712, isoform 1 extracellular domain.
