NPDC-1 (Neural proliferation differentiation and control protein 1) is a 35kD member of the NPDC1/cab1 family of proteins.
It is principally expressed in neurons, and serves as an anti-proliferation agent.
It may accomplish this, in part, by binding to E2F-1 and blocking its protranscriptional activity.
Mature human NPDC-1 is 291aa in length.
It is a type I transmembrane protein that contains an NLS (aa107-124), an HLH domain (aa95-128), a transmembrane domain (aa182-202), and a PEST degradation sequence (aa269-302).
Phosphorylation promotes ubiquitination at this latter site.
There are two splice variants.
One shows a deletion of aa242-263, while another shows a nine aa substitution for the nine aa in positions 218-226.
Over aa35-181, human NPDC-1 shares 68% aa identity with mouse NPDC-1
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.2um filtered solution in PBS, BSA. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA. Labeled with Biotin.
Specificity：Recognizes human NPDC-1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：NS0-derived recombinant human NPDC-1 Preparation, aa35-181.
