Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml NTera-2 human testicular embryonic carcinoma cell lineImmunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human brain (cortex)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:sheep
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug (LAL)
Form:Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:Recognizes human NLGN-4 extracellular domain.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to Met618-Thr673 from human NLGN-4, expressed in E. coli