Human Integrin b7 (also Integrin bP and M290 IEL Ag) is a 120kD member of the Integrin beta chain family of molecules.
It is a type I transmembrane glycoprotein that contains a 704aa extracellular domain (ECD) (aa20-723) and a 52aa cytoplasmic tail.
The ECD contains one von Willebrand factor A domain (aa150-389) and four cysteine-rich EGF-like repeats.
One splice variant exists that shows a deletion of aa501-648.
Integrin b7 forms a nondisulfide linked heterodimer with a4 and aE integrins.
a4b7 binds fibronectin, VCAM-1 and MADCAM1, while aEb7 binds E-cadherin.
The ECD of human b7 is 87% aa identical to the ECD of mouse b7.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.2um filtered solution in PBS, BSA. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA. Labeled with Biotin.
Specificity：Recognizes human Integrin b7 at ~120kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：CHO-cell derived recombinant human Integrin b7 aa22-723; isoform long extracellular domain.
