84-1115-92　Corticoliberin, Pro (Pro-Corticoliberin, Pro-CRF, Pro-CRH, Pro Corticotropin releasing hormone, Pro Corticotropin-releasing factor) 100ug　C7903-80Z

特徴

• Pro-Corticoliberin (also Pro-CRF and Pro-CRH) is a predicted 19kD member of the CRF/Urotensin I family of hormones.
• It is expressed by placental synctiotrophoblasts, zona fasciculata adrenal cells and paraventricular neurons, and is induced by glucocorticoids.
• Pro-Corticoliberin is the precursor to a mature, 41 amino acid amidated Corticoliberin that induces pituitary ACTH and subsequent cortisol production by the adrenal.
• Human Pro-Corticoliberin is 170aa in length (aa25-194).
• If unprocessed, it undergoes N-terminal amidation to generate a polypeptide 168aa in length (aa27-194).
• Secreted Pro-Corticoliberin is both bioactive and binds to 37kD Corticoliberin-PB.
• In the placenta, Pro-Corticoliberin is only occasionally cleaved to generate mature Corticoliberin (aa152-192); in the brain, it undergoes active internal proteolysis to generate multiple fragments, including the 5kD mature peptide.
• Over aa25-196, human Pro-Corticoliberin shares 78% aa identity with mouse pro-Corticoliberin.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/ml ELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.