Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37-43kD member of the S1 peptidase family of enzymes.
In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D.
This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis.
Mature mouse Factor D is 234 amino acids in length (aa26-259).
In contains one peptidase S1 domain that cleaves an Arg-Lys bond (aa26-254).
There are at least two potential alternative splice forms.
One shows a two aa substitution for aa 1-71, and a second shows a 15 aa substitution for aa1-107.
Mature mouse factor D shares 85% and 68% aa identity with rat and human Factor D, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml using recombinant mouse Complement Factor D
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Specificity：Recognizes mouse Complement Factor D. Species Crossreactivity: recombinant human Complement Factor D; ~5%, recombinant human Granzyme A; <1%
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ile26-Ser259 from mouse Complement Factor D, expressed in NSO cells. Species Sequence Homology: rat; 85%, human; 68%
