SDF-1 alpha, otherwise known as CXCL12a, a stromal cell derived CXC chemokine which, together with SDF-1 beta, arises from alternative splicing of the SDF-1 gene, and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons.
SDF-1 binds with high-affinity to the G protein–coupled receptor CXCR4 (fusin), and acts as an inhibitor of the CXCR4-mediated entry of HIV-1 virus into target T-cells.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-2ug/mlWestern Blotting: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:goat
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder in PBS, pH7.2. Reconstitute with 100ul dH20, 0.09% sodium azide.
Specificity:Recognizes mouse SDF-1 alpha.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Mo
Immunogen:Recombinant mouse SDF-1 alpha, expressed in E. coli.