CD184, otherwise known as CXCR4 (C-X-C chemokine receptor type 4), is a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, ubiquitously expressed in blood and tissue cells, which acts as a specific receptor for the chemokine SDF-1 (Stromal cell-derived factor 1).
Interaction of CD184 with SDF-1 mediates the chemotaxis of progenitor and mature blood cells, and is critical for B- lymphopoiesis and myelopoiesis.
CD184 is a major co-receptor for infection by T-cell tropic strains of HIV1, and also a primary receptor for some HIV2 isolates.
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Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:150 ELISA: 1:25,000Western Blot: 1:250Optimal dilutions to be determined by the researcher.
Positive Control:Spleen
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM KHPO4, 140mM sodium chloride, pH7.2, 0.09% sodium azide, 0.1% BSA.
Specificity：Recognizes an epitope within the N-terminal region of human CD184. Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide TEEMGSGDYDSMKEPCFREENANFNKC corresponding to aa17-43 within the N-terminal region of human CD184.
