CCR4 (C-C chemokine receptor type 4) is a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, preferentially expressed by CD4+ Th2 (type 2 helper T) cells, which acts as a high affinity receptor for the chemokines MDC (macrophage-derived chemokine), MCP-1, MIP-1, RANTES and TARC.
Applications:Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 1:20.
Use 10ul to label 1x10e6 cells in 100ulImmunohistochemistry (Frozen): 1:300ELISA (Indirect): 1:50,000 Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, 0.1% BSA, 0.09% sodium azide
Specificity：Recognizes mouse CCR4. Does not crossreact with human CCR4.
Isotype：IgG F(ab’)2
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to aa8-26, DTTQDETVYNSYYFYESMPC from mouse CCR4.
