Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals.
They comprise a group of proteinase inhibitors, widely distributed in tissues and body fluids, and form tight complexes with cysteine proteases such as cathepsin B, H, L and S.
Cystatin C, a secreted molecule of this family, is of interest from biochemical, medical and evolutionary points of view.
Cystatin C, with molecular weight of 13260D, is composed of 120 amino acids, lacks carbohydrate and has two disulfide bridges located near the carboxyl terminus.
Cystatin C is increased in patients with malignant diseases and is related to the insufficiency of renal function and appears to be a better marker than creatinine.
On the other hand, low levels of cystatin C involve cause the breakdown of the elastic laminae and, subsequently, the atherosclerosis and abdominal aortic aneurysm.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000Western Blot: 0.3-1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control:Human Testis and Prostate lysates
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by antigen immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human CST3 at ~16kD.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-SVEEEGVRRALD, from the internal region of human CST3 (NP_000090.1).
