84-1115-53　XRCC6 (X Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6, X-ray Repair Complementing Defective Repair in Chinese Hamster Cells 6, 70kD Subunit of Ku Antigen, ATP Dependent DNA Helicase 2 Subunit 1, ATP-dependent DNA Helicase II 70kD Subunit, CTC Box Binding Factor 75kD Subunit, CTC75, CTCBF, DNA Repair Protein XRCC6, G22P1, Ku 70, Ku70, Ku-70, Ku Autoantigen 70kDa, Lupus K ...　X9506-05J

特徴

• Originally identified as an autoantigen, the Ku proteins are nuclear proteins found in eukaryotes.
• They exist as a heterodimer consisting of Ku70 and Ku80, and migrate at approximately 70 and 80kD, respectively.
• They bind to DNA ends at double-strand breaks.
• The Ku proteins interact with the DNA-dependent protein kinase (DNA-PK), and participate in the targeting of DNA-PK to DNA.
• The Ku proteins may participate in numerous cellular processes, including DNA repair and replication, cell signaling, and proliferation, transcriptional activation, and apoptosis.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:2000Western Blot: 0.1-0.3ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Lysates of cell lines A431, HeLa and HepG2
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 monthsat -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
• See X9506-05J-P.
• Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
• When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
• To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
• Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
• It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
• Then incubate and develop the two blots in parallel.