84-1115-52　WISP1 (Wnt 1 induced Secreted Protein 1, Wnt-1-induced Secreted Protein 1, Wnt1-induced Secreted Protein 1, WISP 1, WISP-1, CCN4, WISP1c, WISP1i, WISP1tc, Wnt-1-induced Secreted Protein, Wnt1-induced Secreted Protein, Wnt-1-inducible Signaling Pathway Protein 1, Wnt1-inducible Signaling Pathway Protein 1, Wnt 1 Signaling Pathway Protein 1, Wnt1 Signaling Pathway Protein 1, Wnt-1 Signaling Path ...　W1450-50A

特徴

• This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family.
• WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes.
• The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain.
• This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation.
• It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors.
• The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation.
• It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase.
• It is 83% identical to the mouse protein at the amino acid level.
• Alternative splicing of this gene generates 2 transcript variants.
• Applications:Suitable for use in ELISA and Western Blot.
• Other Applications not tested.
• Recommended Dilutions:ELISA: 1:128,000Western Blot: 0.1-0.3ug/ml
• Optimal dilutions to be determined by the researcher
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 monthsat -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
• See W1450-50A-P.
• Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
• When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
• To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
• Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
• It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
• Then incubate and develop the two blots in parallel.