PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C).
The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits.
There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72 .
The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma .
The B' (or B56) family consists of at least seven splice variants derived from five genes.
RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene.
Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals.
PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism.
PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid.
It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.
Applications:Suitable for use in ELISA and Western Blot.
Other Applications not tested.
Recommended Dilutions:ELISA: 1:2000Western Blot: 2-3ug/mlOptimal dilutions to be determined by the researcher
Positive Control:Human Brain (Cerebellum) lysates
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 monthsat -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
See P9107-34U-P.
Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
Then incubate and develop the two blots in parallel.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human PP2A-B’ (B56) at ~55kD. Species sequence homology: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide, C-QRLTPQVAASGGQS, from the C-Terminus of human PP2A-B’ (B56).
