Group IB secretory phospholipase A2 (sPLA2-IB) mediates cell proliferation, cell migration, hormone release and eicosanoid production via its receptor in peripheral tissues.
In the CNS, high-affinity binding sites of sPLA2-IB have been documented.
sPLA2-IB induced neuronal cell death in a concentration dependent manner depending on PGD2 metabolites, especially Delta12-PGJ2 that might mediate sPLA2-IB-induced apoptosis.
The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Peptide ELISA Titer: 1:64,000Western Blot: 0.01ug/ml; ~17-18kD band observed in Human Pancrease lysates (calcuated MW: 16.4kD according to NP_000919.1).
In transfected HEK293 transiently expressing PLAG1B a band of ~18kD is observed.
This band is not observed in the non-transfected HEK293
Optimal dilutions to be determined by the researcher.
Positive Control:Human Pancreas lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human Phospholipase A2, Secretory, Group IB. Species sequence homology: canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to NKAHKNLDTKKYCQS, from the C-Terminus of human Phospholipase A2, Secretory, Group IB (NP_000919.1).
