84-1115-28　NudE-L (NudE-like Protein, Nudel, Nuclear Distribution Gene E Like Homolog 1, NDEL1, DKFZp451M0318, Endooligopeptidase A, EOPA, Mitosin-associated Protein 1, Mitosin-associated Protein MITAP1) 100ug　N7400-30D

特徴

• Nudel, or endooligopeptidase A, a protein highly expressed in the brain where it associates with dynein motors and Lis1, both of which have been linked to neuronal development and migration.
• Nudel facilitates the polymerisation of neurofilaments and the organisation of the cellular microtubule array and microtubule anchoring at the centrosome.
• It is required for several dynein- and microtubule-dependent processes such as the centripetal motion of secretory vesicles, coupling of the nucleus and centrosome and maintaining Golgi integrity.
• Nudel also serves as a Cdk5 substrate and its phosphorylation status affects its localisation in neurons.
• Applications:Suitable for use in ELISA and Western Blot.
• Other Applications not tested.
• Recommended Dilutions:ELISA: 1:8000Western Blot: 0.1-0.3ug/mlOptimal dilutions to be determined by the researcher
• Positive Control:Human Brain (Cerebellum) lysates
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 monthsat -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
• See N7400-30D-P.
• Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
• When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
• To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
• Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
• It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
• Then incubate and develop the two blots in parallel.