This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane.
This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex.
The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor.
Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML).
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other Applications not tested.
Recommended Dilutions:ELISA: 1:32,000Western Blot: 0.01-0.03ug/mlImmunohistochemistry (Paraffin): 2-3ug/ml Requires HEIR using citrate buffer pH6.0Optimal dilutions to be determined by the researcher.
Positive Control:Human lysates of cell line A431
Blocking Peptide:N5385-01A1
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 monthsat -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human NRAS at ~21kD. Species sequence homology: mouse, rat, canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide, C-RMKKLNSSDDGTQ, from the internal region of human NRAS.
