~70kD band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 67.7kD according to Human NP_004813.1 and 67.8kD according to Rat NP_446183.1).
Optimal dilutions to be determined by the researcher.
Peptide Blocking:N1193-16C-P: Netrin 1, corresponding peptide; Peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration is 0.5mg/ml.
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20 minutes.
Then incubate and develop the two blots in parallel.
Recommended Positive Control:Human Brain (Cerebellum), Mouse and Rat Brain lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes rat Netrin 1 at ~70kD. Species Crossreactivity: human, mouse. Species sequence homology: canine, bovine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide KFQQREKKGKCKKA from the C-Terminus of human Netrin 1.
