Endocannabinoids, such as arachidonyl ethanolamide (AEA) and 2-Arachidonyl glycerol (2-AG), function as short-range modulators of cell and synaptic activity.
Monoacylglycerol lipase (MAGL) hydrolyzes 2-AG to terminate its biological actions and works consecutively with hormone-sensitive lipase (HSL) to mobilize fatty acids from the triglyceride stores of adipocytes.
MAGL has a molecular weight of ~33kD and exhibits a high degree of homology among human, mouse, and rat at the amino acid level.
MAGL is expressed in a variety of tissues such as kidney, spleen, heart, liver, testis, stomach, brain, lung, and adrenal gland, with most abundant expression in skeletal muscle and adipose tissue.
This suggests a role of MAGL in monoglyceride hydrolysis in diverse tissues.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:32,000Western Blot: 0.5-1.5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control:Human Adipose and Brain (Frontal Cortex) lysates
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 monthsat -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
See M4529-96A-P.
Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
Then incubate and develop the two blots in parallel.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human MGLL at ~30kD. Species sequence homology: mouse, rat, canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide, C-QDLPHLVNADGQY, corresponding to human MGLL.
