84-1115-10　HADH (hydroxyacyl-Coenzyme A dehydrogenase, HAD, HADH1, HADHSC, HCDH, HHF4, Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial precursor, L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain, M/SCHAD, Medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase, MGC8392, SCHAD, Short chain 3-hydroxyacyl-CoA dehydrogenase, Short chain 3-hydroxyacyl-CoA dehydrogenase, mitochondrial precurso ...　H1500-01A

特徴

• Short-chain L-3-hydroxyacyl-CoA dehydrogenase (SCHAD; EC1.1.1.35) plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids.
• It catalyzes the reversible dehydrogenation of 3-hydroxyacyl-CoAs to their corresponding 3-ketoacyl-CoAs with concomitant reduction of NAD to NADH and exerts it highest activity toward 3-hydroxybutyryl-CoA.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:64,000Western Blot: 0.01-0.03ug/ml Optimal dilutions to be determined by the researcher.
• Positive Control:Human Heart, Muscle and Kidney lysates
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 monthsat -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
• See H1500-01A-P.
• Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
• When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
• To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
• Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
• It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
• Then incubate and develop the two blots in parallel.