Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors.
Alternative splicing of this gene results in two products.
Might normally function as a repressor.
EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process.
They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex.
They may also contribute to an aberrant activation of the fusion protein target genes.
Applications:Suitable for use in ELISA and Western Blot.
other applications not tested.
Recomended Dilutions:ELISA: 1:16,000Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher
Positive Control:Nuclear lysates
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 monthsat -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
See E9002-85F-P.
Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
Then incubate and develop the two blots in parallel.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human EWS at ~90kD. Species sequence homology: mouse, rat, bovine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide, C-TSYDQSSYSQQNTYG, from the internal region of human EWS.
