84-1115-03　CLEC16A (C-type Lectin Domain Family 16, Member A, Gop-1, KIAA0350, KIAA0350 Protein, MGC111457, Uncharacterized Protein KIAA0350) 100ug　C5839-74A

特徴

• Applications:Suitable for use in Peptide ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Peptide ELISA: 1:1000Western Blot:1-3ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Human Kidney and Liver lysates
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
• See C5839-74A-P.
• Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
• When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
• To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
• Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
• It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
• Then incubate and develop the two blots in parallel.