84-1114-91　AKAP6 (A Kinase (PRKA) Anchor Protein 6, ADAP6, PRKA6, mAKAP, ADAP100, AKAP100, KIAA0311, A-Kinase Anchor Protein 100) 100ug　A1102-81E

特徴

• The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell.
• This gene encodes a member of the AKAP family.
• The encoded protein is highly expressed in various brain regions and cardiac and skeletal muscle.
• It is specifically localized to the sarcoplasmic reticulum and nuclear membrane, and is involved in anchoring PKA to the nuclear membrane or sarcoplasmic reticulum.
• Applications:Suitable for use in ELISA.
• Other Applications not tested.
• Recommended Dilutions:ELISA: 1:16,000Optimal dilutions to be determined by the researcher
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 monthsat -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Peptide Blocking:Corresponding peptide is available for Peptide Blocking studies.
• See A1102-81E-P.
• Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet.
• When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml.
• To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed).
• Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature.
• It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min.
• Then incubate and develop the two blots in parallel.