Hemoglobin is composed of one globin plus four hemes; heme consists of protoporphyrin IX and ferrous iron.
The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520; however, ultracentrifugal and osmotic pressure measurements indicate an actual molecular weight four times the minimum molecular weight.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Capture ELISA: 5ug/ml Western Blot: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human Hemoglobin. Forms a single precipitin band with human adult hemoglobin A1, A2, F and S, by immunoelectrophoresis.
