84-1114-66　［取扱停止］JAK2 (Janus Kinase 2) 100ug　J0901-11

特徴

• The Janus (JAK) family of cytoplasmic tyrosine kinases has been implicated in signal transduction induced by cytokines and growth factors, physically associated with ligand-bound receptors.
• This association that results in tyrosine phosphorylation and activation.
• JAK1 is approximately 130kD and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members.
• Family members such as JAK1, JAK2, and TYK2 are ubiquitous, whereas JAK3 is predominantly expressed in T lymphocytes.
• Studies with mutant cells that do not express JAK1, JAK2, or TYK2 show that their activation is essential for cellular signaling.
• JAK family kinases may either phosphorylate each other or be phosphorylated by a yet undescribed tyrosine kinase.
• Different cytokines can activate via phosphorylation distinct JAK family members.
• In some cells, the membrane-associated gp130 protein has been implicated in the induction of JAK family phosphorylation, although gp130 itself has no known activity.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommened Dilutions:ELISA: 1:64,000Western Blot: 1:2000 detects JAK2 in TF-1 cell extract at ~130kD
• Optimal dilutions to be determined by researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.