c Kit, also known as CD117 and stem cell factor receptor, is a 145kD transmembrane tyrosine kinase encoded by the c Kit proto-oncogene.
c Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion.
Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation.
c Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer.
Phosphorylation at tyrosine 936 of c Kit allows binding of Grb2 and activation of the Ras, Raf, ERK1&2 signaling pathway.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:64,000, peptide coated at 100ng/well
Other applications not tested.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, ddH2O.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from 0.01M PBS, pH 7.4. Reconstitute with 100ul of sterile ddH2O.
Specificity：Recognizes the synthetic, phosphorylated, human c-Kit (Tyr719) peptide.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (SDSTNEYpMDMKPG) derived from the human phosphorylated c-kit (Tyr719) (KLH).
