Bcl-XL (28kD) is part of the Bcl-2 family, which are important regulators of apoptosis.
Bcl-X has two isoforms, Bcl-XL (long) a 241 amino acid protein and shares significant homology with Bcl-2.
Bcl-XS (short) a 178 amino acid protein lacking a 63 amino acid domain near the transmembrane region that is well conserved among members of the Bcl-2 family.
Like Bcl-2, Bcl-XL has the ability to inhibit apoptosis and is widely studied for its role in tumor generation.
In a recent study, analysis of Hodgkin’s disease showed that 71% of cases (114/160) showed immunohistochemical reactivity with Bcl-XL in Reed-Sternberg cells.
In some cases, Bcl-XL reactivity was present in Reed-Sternberg cells that were negative for CD15 and CD30.
Atypical hyperplasias (0/28) were negative for Bcl-XL.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000 for detection of coated peptide (100ng/well)Optimal dilutions to be determined by researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, ddH2O.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from 0.01M PBS, pH 7.4. Reconstitute with 100ul of sterile ddH2O.
