he remodelling of the primitive vasculature in mammals to form the mature cardiovascular system includes a number of factors directing endothelial cell development.
Angiopoietin-1 is an angiogenic secreted protein that interacts with the endothelial specific Tie-2 receptor.
It is primarily expressed in developing endothelial cells.
During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2.
Ang-1 appears to play a crucial role in mediating matrix and mesenchyme.
It mediates blood vessel maturation and stability.
Ang-1 may play a critical role in heart development.
Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2.
A homolog of Ang-1, termed Angiopoietin-2 may act as an antagonist for Ang-1 and Tie-2.
Ang-1 and Ang-2 have ~60% sequence homology.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes mouse Angiopoietin-1. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide selected from mouse Angiopoietin-1 (KLH). Cellular Localization: Secreted. Species sequence homology: bovine, canine, porcine, equine (95%).
