Stella was initially identified in primordial germ cells and pre-implantation embryos whose expression as a maternal factor is important in early embryonic development but is not required for germ cell specification in mice.
In humans, Stella is thought to be a marker for pluripotency in embryonic stem (ES) cells as its expression is observed in primordial germ cells of both sexes and germ cell tumors but not in normal somatic tissues.
However, in ES cell colonies, heterogeneous expression of Stella was seen in high throughput in situ hybridization assays, indicating that higher levels of complexity exist in otherwise thought to be undifferentiated ES cells.
At least two distinct isoforms of Stella are known to exist.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control: 293 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：PBS containing 0.02% sodium azide.
Specificity：Recognizes human Stella. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 13aa from near the carboxy terminus of human Stella.
