Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neural transmission and plasticity (1,2).
While both Rim1 and Rim 2 are thought to be effector proteins for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner, less is known of Rim3.
Expression of Rim3 in PC12 cells induced a significant increase in calcium-triggered exocytosis, with no appreciable change in the baseline release, suggesting that it plays a role in the regulation of exocytosis (1).
Rim3 protein localizes primarily to neuronal dendrites and the postsynaptic densities, as opposed to Rim1 which is found in presynapse locations, indicating that Rim3 may contribute to synapse transmission and plasticity (2).
This antibody is predicted to have no cross-reactivity to other Rim proteins.
Applications:Suitable for use in Elisa and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5–1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Rim3. Species crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 17aa from near the amino terminus of human Rim3 (GenBank accession no. NP_055562).
