Phosphoribosyl transferase domain containing 1 (PRTFDC1) is highly homologous to the hypoxanthine phosphoribosyltransferase (HPRT1) and may have arisen from a gene duplication event of a common ancestor gene.
Recently, it was shown that CpG islands in the PRTFDC1 promoter could be hypermethylated in ovarian cancers and oral squamous-cell carcinomas (OSCC), leading to gene silencing.
Restoration of PRTFDC1 expression in OSCC inhibited cell growth in colony-formation assays, while knockdown of PRTFDC1 expression in OSCC that expressed the gene promoted cell growth.
These results suggest that PRTFDC1 can act as a tumor-suppressor gene.
At least three isoforms of PRTFDC1 are known to exist.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Brain Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human PRTFDC1. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 14aa from near the center of human PRTFDC1.
