Apoptosis is related to many diseases and development.
Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators.
Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9.
Activated caspase-9 cleaves and activates the effector caspases, which are responsible for the proteolytic cleavage of many key proteins in apoptosis.
The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis.
PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway.
PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000Western Blot: 0.2-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes ANP32B at ~30 kD in A431 and human lymph node lysates (predicted MW 29kD according to NP_006392). This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741. Species Crossreactivity: Mouse and rat.
