Proteins involved in MDR mechanisms are P-glycoprotein (Pgp), Multidrug Resistance-associated Protein (MRP) and Lung Resistance-related Protein (LRP).
Pgp and MRP belong to the ATP binding cassette (ABC) superfamily of membrane transporter proteins.
This superfamily comprises a broad range of proteins found in organisms from bacteria to humans, which transport a wide variety of substances such as ions, amino acids, sugars, peptide and proteins.
Although increased levels of Pgp are likely to contribute to MDR in at least some tumor types, it has become evident that alternative, non-Pgp-mediated mechanisms of MDR exist.
The multidrug resistance-associated protein (MRP1) has been identified by Cole and Deeley.
The amino acid identity between MRP1 and Pgp is only 15%, and the homology to chloride channel CFTR only 19%.
Paulusma, et al., cloned the cDNA for rat canalicular multispecific organic anion transporter (cMOAT or MRP2) which turned out to be a novel liver specific homolog of human MRP1.
Recently, by screening database of human expressed sequences tags, Kool identified three new homologs of MRP1, called MRP3, MRP4 and MRP5.
Several excellent reviews appeared recently dealing with mechanisms of cellular drug resistance.
In addition to MDR-related proteins many other mechanisms of drug resistance have been documented in vitro.
They are based on alterations in drug target enzymes and proteins, increased detoxification, alterations in cellular metabolism, enhanced ability to repair DNA damage, and failure to undergo apoptosis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:64000Western Blot:1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline 0.5mg/ml, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human ABCC4 at ~170kD in rat renal cortex membranes (calculated MW of 149kDa according to Rat NP_596902.1 and 150kD according to Human NP_005836.2). This antibody is expected to recognize isoform 1 (NP_005836.2) only. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mk Rt
Immunogen：Synthetic peptide of ABCC4.
Shelf Life：1year
EU Commodity Code：30021010
商品名：MRP4 (Multidrug Resistance-associated Protein 4, ATP-binding Cassette Sub-family C (CFTR/MRP) Member 4, ATP Binding Cassette Sub-family C Member 4, ABCC4, bA464I2.1, Canalicular Multispecific Organic Anion Transporter ABC Superfamily, Canalicular Multispecific Organic Anion Transporter, EST170205, MRP/cMOAT Related ABC Transporter, Multispecific Organic Anion Transporter B, MOATB, RP11-74A12.1) 100ug
