The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins.
Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains.
Alpha 4 chain associates with either beta 1 or beta 7 chain.
It has been demonstrated that the putative ligand-binding sites of both integrin alpha 4 beta 1 and alpha 4 beta 7 is located on the alpha 4 chain.
These ligands included Madcam, VCAM, and fibronectin.
Madcam is known as the principal ligand for integrin alpha 4 beta 7.
Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha 4 beta 7, the gut mucosal homing receptor for peripheral T cells.
Despite its predicted molecular weight, Integrin alpha 4 will often run at higher molecular weight in SDS-PAGE.
At least two isoforms are known to exist.
The immunogen to this antibody is outside of the Integrin alpha 4 recombinant protein fragment and thus will not recognize it.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Integrin alpha 4. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 13aa from near the carboxy terminus of human Integrin alpha 4.
