84-1114-09　Glutathione S-Transferase, pi (GSTP1, GSTpi1, GST3, DFN7) 100ug　G8135-25C

特徴

• Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione.
• Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta (reviewed in 1).
• The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism (i.e., the metabolism of environmental mutagens and carcinogens) and may play a role in susceptibility to cancer (2).
• More recent experiments have suggested that differential expression of GSTP1 also contributes to the sensitivity of xenobiotics in the substantia nigra and may influence the pathogenesis of reactive oxygen species-induced neurological disorders such as Parkinson's disease (3).
• CpG island hypermethylation of the GSTP1 promoter leading to the silencing of the GSTP1 gene has also been linked to cancer (4).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5–1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.